Optimized Production and Characterization of ?-fructofuranosidase from Penicillium brevicompactum

Copper uptake by Penicillium brevicompactum.

The copper binding properties of Penicillium brevicompactum biomass were influenced by growth phase of mycelium and concentration of copper in reaction mixtures. The efficiency of copper uptake increased with growth time and was largest at the mid-logarithmic growth phase. The time course of copper uptake was biphasic. Double reciprocal plots of absorption velocity of copper vs. copper concentr...

Production of mycophenolic acid by Penicillium brevicompactum—A comparison of two methods of optimization

Production of mycophenolic acid (MPA) by submerged fermentation using the microfungus Penicillium brevicompactum MTCC 8010 is reported here. Screening experiments were used to identify: the suitable media composition; the optimal initial pH; and the optimal incubation temperature to maximize the production of MPA in batch cultures. The initial concentrations of the selected sources of carbon (g...

Characterization of recombinant β-fructofuranosidase from Bifidobacterium adolescentis G1

BACKGROUND We have previously reported on purification and characterization of beta-fructofuranosidase (beta-FFase) from Bifidobacterium adolescentis G1. This enzyme showed high activity of hydrolysis on fructo-oligosaccharides with a low degree of polymerization. Recently, genome sequences of B. longum NCC2705 and B. adolescentis ATCC 15703 were determined, and cscA gene in the both genome seq...

Molecular identification of MPaB and MPaE genes from MPA gene cluster in new strain of Penicillium brevicompactum

Mycophenolic acid (MPA) is a fungal metabolite possessing antiviral, antifungal, antibacterial, antitumor and anti-psoriasis activities. It is being used as an immunosuppressive agent in kidney, heart and liver transplantation patients. In the presence of MPA, the proliferation of the B and T lymphocytes is inhibited. The MPaB and MPaE genes reside in a 25 kb gene cluster in t...

Molecular and Biochemical Characterization of a -Fructofuranosidase from Xanthophyllomyces dendrorhous †